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The cleaned milled grains were ground in an Udy Cyclone Mill (Udy Corp., Fort Collins, USA) with a 1.0 mm mesh screen. Amylose content was determined by Auto-Analyzer (Bran + Luebbe ; Germany) with an Automated Analyzer Control and Evaluation software for Windows Ver. 5.30 (2001) using the method of Juliano (1971). 2.2.4.

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The prolamin compositions of the F3 samples were also confirmed. Quality evaluation F~ grains from each F2 plant were milled using a Udy Cyclone Mill (Tecator AB, Sweden) equipped with a 1.0-mm sieve. Gluten strength was es- timated by the modified SDS-sedimentation (SDSS) test~9 with some minor modifications2.

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Physical characteristics of the grain were measured using a 1625 Grain Inspector (Foss, Denmark). In order to measure amylose and gel consistency, polished grains were ground to pass through a 0.5 mm sieve in a cyclone mill (Udy Cyclone Sample Mill 3010-030, Fort Collins, CO).

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